This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 290 of mouse caspase-9
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) affinity purified rabbit anti-caspase-9 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Alternative names of antibody target:
CASP-9 ,EC 22.214.171.124 ,ICE-like apoptotic protease 6 , ICE-LAP6,Apoptotic protease Mch-6 ,Apoptotic protease-activating factor 3 , Apaf-3
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 45 kDa procaspase-9, 35/37 kDa cleaved immediate fragments and the 17-25 kDa fully cleaved caspase-9.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
Caspase family of cysteine proteases plays a key role in apoptosis. Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis, cytochrome c released from mitochondria associates with procaspase-9/Apaf-1. The complex processes procaspase-9 into a large subunit (35 kDa or 17-25 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage, procaspase-9 can also be cleaved in vivo by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.