This antibody reacts with:
Affinity Purified Antibody
Human recombinant Caspase-10/a (Cat # 1090A)
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
30 µg (0.5 mg/ml) of Caspase-10 antibody in PBS pH 7.2, 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol.
Alternative names of antibody target:
FAS-associated death domain protein interleukin-1B-converting enzyme 2, FLICE2, ICE like Apoptotic Protease 4
Western blot: 1-4 µg
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
Caspase family of cysteine proteases plays a key role in apoptosis. Caspase-10 is expressed in many tissues and cell lines. Similar to other caspases, caspase-10 is also synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis. Four isoforms of caspase-10 have been identified: caspase-10a (Mch4), caspase-10b (FLICE2), caspase-10c and caspase-10d. They have the same prodomain but different mature large and small subdomains. Activated caspase-10 activates caspase-3,-4,-6,-7,-8, and –9, which are then involved in proteolytic cleavage of many cellular proteins. Caspase-10 has been shown to be involved in the Fas and TNFR1 - induced apoptosis.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.