This antibody reacts with:
Human, mouse, rat, bovine, pig, rabbit
Synthetic peptide surrounding amino acid 700 of rat Calpain 1
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-calpain 1 (large subunit) antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Alternative names of antibody target:
EC 184.108.40.206, CAPN2
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The anti-calpain I antibody recognizes the large subunit (80 kDa) of human Calpain 1. It does not recognize the smaller subunit of human Calpain 1.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Type I calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.