This antibody reacts with:
E. coli-expressed recombinant human c-Jun fusion protein
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-human c-Jun polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5mM EDTA and 0.01% thimerosal.
Alternative names of antibody target:
JUN , AP1 , p39 , c-Jun
The antibody can be used in Western Blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human c-Jun (Cat.# 7001-100) can be used as a positive control.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins, and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. The MAP kinase homologue, JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. The activity of JNK is stimulated by the same signals that activate c-Jun.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.