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anti-BTF Antibody

  • Catalog number
  • Product name
    anti-BTF Antibody
  • Size
    BTF Antibody
  • Price
  • Supplier
    biovision antibodies-kits-proteins
  • Ask
  • Antibody host:
  • This antibody reacts with:
    Human, mouse
  • Purification method:
    Affinity purified
  • Immunogene sequence:
    Synthetic peptide of human BTF
  • How to handle this antibody:
    The antibody solution should be gently mixed before use.
  • Form of antibody:
  • This antibody has been succesfully tested for use in following methods:
    Western blot, Immunoprecipitation
  • Target of this antibody:
  • Formulation of this antibody:
    100 µl purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
  • Alternative names of antibody target:
    BTF, BCLAF1, BCLAF-1, BCL2-Associated Transcription Factor 1, KIAA0164
  • Recommended usage:
    Western blotting (1:500-1:2000) and immunoprecipitation. However, the optimal concentrations should be determined individually. HeLa cell lysate can be used as a positive control for Western blotting. The antibody recognizes the BTF of human and mouse origins. Reactivity to other species has not been tested.
  • Storage information:
    Shelf time: 12 months in temp.: -20 deg.C
  • Shipping conditions:
    gel pack
  • Clonality of antibody:
  • Isotype:
    Rabbit IgG
  • Background information:
    The initiation of gene transcription involves the ordered assembly of a multiprotein complex on proximal promoter elements such as the TATA box. In addition to RNA polymerase II, the transcription factors class II (TFII) family of proteins are required for initiation of transcription, as the first step in the formation of this initiation complex is the stable binding of TFIID to the TATA box. An additional TFII related protein, BTF3, does not directly associate with the proximal promoter, but rather forms a stable complex with RNA pol II and facilitates RNA pol II assembling into the complex. The BTF3 gene is ubiquitously expressed and encodes for two protein isoforms, BTF3a and BTF3b, which are produced from alternative splicing. The BTF3 proteins are identical except that BTF3b lacks the first 44 amino acids at the N-terminal of BTF3a. As a consequence of this deletion, BTF3b is unable to induce transcription, despite being able to bind RNA pol II. Additionally, BTF3a and BTF3b associate with the widely expressed protein kinase CK2. CK2 phosphorylates BTF3a, as well as TFIIB, and is required for the efficient transcription of the tRNA and 55 rRNA genes by RNA pol III. BTF3 belongs to the NAC-β family, which includes several related proteins, such as BTF3L1, BTF3L2, BTF3L3 and BTF3L4.
  • Concentration of antibody:
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Gene target
  • Short name
    anti-BTF Antibody
  • Technique
    Antibody, antibody to, antibodies against human proteins, antibodies for
  • Alternative name
    antibody to-BTF (Antibody to)
  • Alternative technique
  • Ask
1. Gene info
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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