This antibody reacts with:
E. coli expressed recombinant mouse BLC
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) Protein A purified rabbit anti-BLC polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Alternative names of antibody target:
Western blot (at 0.5-4 µg/ml). However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
BLC (B-lymphocyte chemoattractant) is a 12 kDa ELR-CXC chemokine. BLC showed high levels of expression in spleen, Peyer’s patches, and mesenteric lymph nodes. BLC is reported as the ligand of CXCR5. It attracts primarily B cells in vitro. Cell lines transfected with CXCR5 demonstrate both chemotaxis and calcium flux in response to BLC.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.