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anti-Bcl-2 Antibody (Clone Bcl-2/100)

  • Catalog number
    3195-100
  • Product name
    anti-Bcl-2 Antibody (Clone Bcl-2/100)
  • Size
    Bcl-2 Antibody (Clone Bcl-2/100)
  • Price
    360EUR
  • Supplier
    biovision antibodies-kits-proteins
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  • Antibody host:
    Mouse
  • This antibody reacts with:
    Human
  • Purification method:
    Affinity purified
  • Immunogene sequence:
    Synthetic peptide corresponding to resides surrounding a.a. 44 of Human Bcl-2
  • How to handle this antibody:
    The antibody solution should be gently mixed before use.
  • Form of antibody:
    Liquid
  • This antibody has been succesfully tested for use in following methods:
    Western blot, Immunoprecipitation, Immunohistochemistry
  • Target of this antibody:
    Bcl-2
  • Formulation of this antibody:
    100 µg (0.5 mg/ml) protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95%. The antibody was formulated in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol
  • Alternative names of antibody target:
    BCL2, BCL 2
  • Recommended usage:
    Western blot (1-4 µg/ml), Immunoprecipitation, Immunohistochemistry, and Flow cytometry. However the optimal conditions should be determined individually. Recognize the 26 kDa human Bcl-2
  • Storage information:
    Shelf time: 12 months in temp.: -20 deg.C
  • Shipping conditions:
    gel pack
  • Clonality of antibody:
    Monoclonal
  • Isotype:
    Mouse IgG1
  • Clone:
    Bcl-2/100
  • Background information:
    Bcl-2 (B-cell leukemia/lymphoma 2) gene was discovered by walking the chromosomal breakpoints t(14;18). This gene consists of two exons and several transcripts that encode two different protein products, Bcl-2α and Bcl-2β. Both proteins are approximately 26-30 kDa and differ at their C-terminal region. Bcl-2 suppresses apoptosis (programmed cell death) and this property appears to be modulated by homodimerization or association with Bax and Bad, two cell death promoters
  • Concentration of antibody:
    0.5 mg/ml
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Gene target
  • Short name
    anti-Bcl-2 Antibody (Clone Bcl-2/100)
  • Technique
    Antibody, antibody to, antibodies against human proteins, antibodies for
  • Alternative name
    antibody to-Bcl-2 (Antibody to) (clonality Bcl-2/100)
  • Alternative technique
    antibodies
  • Clone name
    Bcl-2
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MESH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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