His-tag polyclonal antibody

Alternative Name

6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
Immunogen

Synthetic peptide His-tag.
Swiss Prot

N/A
Reactivity

All
Applications

WB
Application_all

WB: 1:500~1:1000
Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity

His-tag polyclonal antibody detects over-expressed or recombinant proteins containing the 6× His epitope tag
Usage

Western blot (WB) analysis of over-expressed His-tagged protein in E. coli, the antibody dilutions are 1:2000 (lane 1) and 1:5000 (lane 2). Each lane was loaded with 10 μg of E. coli lysate.
BiowMW

N/A
Note

For research use only, not for use in diagnostic procedure.
Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Conjugation

histidine
Group

Polyclonals and antibodies
About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation

anticorps

Gene target

His-tag
Short name

His-tag polyclonal antibody
Technique

Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Tags
Host

Rabbit
Label

His
Alternative name

histidine-detection labelled polyclonal (antibody to-)
Alternative technique

polyclonals, antibodies

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data