This antibody reacts with:
Human, mouse, rat, bovine
Synthetic peptide corresponding to residue surrounding amino acid 80 of human Akt
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Western blot,Immunoprecipitation, Immunohistochemistry
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-Akt polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Alternative names of antibody target:
PKB, RAC, Akt 1, Akt 2, Akt 3
Western blotting (0.5-4 µg/ml), in immunoprecipitation (10-40 µg/ml), and Immunohistochemistry (10-40 µg/ml). However, the optimal conditions should be determined individually. The antibody detects Akt1, Akt2 and Akt3.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
Akt, also referred to as PKB and RAC, is the cellular homologue of the viral oncogene v-Akt. This protein kinase is activated by insulin and various growth factors and functions in a pathway involving PI3 kinase as activation is sensitive to wortmanin. Recent evidence sμggests that Akt functions to promote cell survival by actively inhibiting apoptosis.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.