anti-Akt/PKB Antibody

  • Catalog number
    3247-100
  • Product name
    anti-Akt/PKB Antibody
  • Size
    Akt/PKB Antibody
  • Price
    426.53
  • Supplier
    biovision antibodies-kits-proteins
  • Antibody host:
    Rabbit
  • This antibody reacts with:
    Human, mouse, rat, bovine
  • Purification method:
    Affinity purified
  • Immunogene sequence:
    Synthetic peptide corresponding to residue surrounding amino acid 80 of human Akt
  • How to handle this antibody:
    The antibody solution should be gently mixed before use.
  • Form of antibody:
    Liquid
  • This antibody has been succesfully tested for use in following methods:
    Western blot,Immunoprecipitation, Immunohistochemistry
  • Target of this antibody:
    Akt/PKB
  • Formulation of this antibody:
    100 µg (0.5 mg/ml) affinity purified rabbit anti-Akt polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
  • Alternative names of antibody target:
    PKB, RAC, Akt 1, Akt 2, Akt 3
  • Recommended usage:
    Western blotting (0.5-4 µg/ml), in immunoprecipitation (10-40 µg/ml), and Immunohistochemistry (10-40 µg/ml). However, the optimal conditions should be determined individually. The antibody detects Akt1, Akt2 and Akt3.
  • Storage information:
    Shelf time: 12 months in temp.: -20 deg.C
  • Shipping conditions:
    gel pack
  • Clonality of antibody:
    Polyclonal
  • Isotype:
    Rabbit IgG
  • Background information:
    Akt, also referred to as PKB and RAC, is the cellular homologue of the viral oncogene v-Akt. This protein kinase is activated by insulin and various growth factors and functions in a pathway involving PI3 kinase as activation is sensitive to wortmanin. Recent evidence sμggests that Akt functions to promote cell survival by actively inhibiting apoptosis.
  • Concentration of antibody:
    0.5 mg/ml
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Gene target
  • Short name
    anti-Akt/PKB Antibody
  • Technique
    Antibody, antibody to, antibodies against human proteins, antibodies for
  • Alternative name
    antibody to-Akt/PKB (Antibody to)
  • Alternative technique
    antibodies
1. Gene info
  • Identity
  • Gene
  • Long gene name
    AKT serine/threonine kinase 1
  • Synonyms gene name
    • v-akt murine thymoma viral oncogene homolog 1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1986-01-01
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Pleckstrin homology domain containing
  • Havana BLAST/BLAT
  • Locus Specific Databases
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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