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AKT1/3 Antibody, Cy3 Conjugated

  • Long name
    AKT1/3 Polyclonal Antibody, Cy3 Conjugated
  • Category
    Conjugated Primary Antibodies
  • Conjugation
    Cy3
  • Host Organism
    Rabbit (Oryctolagus cuniculus)
  • Target Antigen
    AKT1/3
  • Specificity
    This is a highly specific antibody against AKT1/3.
  • Modification
    Unmodified
  • Modification site
    None
  • Clonality
    Polyclonal
  • Clone
    Polyclonal antibody
  • Concentration
    1ug per 1ul
  • Immunogen_range
    445-480/480
  • Subcellular location
    Cytoplasm, Nucleus
  • Source
    KLH conjugated synthetic peptide derived from human AKT1
  • Gene ID number
    207
  • Swiss Prot
    P31749
  • Tested applications
    IF(IHC-P)
  • Recommended dilutions
    IF(IHC-P)(1:50-200)
  • Crossreactivity
    Human, Mouse, Rat
  • Crossreactive species details
    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Antigen background
    AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
  • Purification method
    This antibody was purified via Protein A.
  • Storage conditions
    Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
  • Excitation Emission
    512,550nm/570,615nm
  • Synonyms
    AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; Protein kinase B; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2; PKBG
  • Properties
    If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the AKT1/3 Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
  • Additional conjugation
    cy3 conjugation kit
  • French translation
    anticorps
  • Gene target
  • Short name
    AKT1/3 Antibody, Conjugated
  • Technique
    Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
  • Label
    Cy3
  • Alternative name
    v-akt murine thymoma viral oncogene homolog 1/3 (Antibody to), cyanine 3 coupled
  • Alternative technique
    antibodies
  • Alternative to gene target
    v-akt murine thymoma viral oncogene homolog 1, AKT and CWS6 and PKB and PKB-ALPHA and PRKBA and RAC and RAC-ALPHA, AKT1 and IDBG-22709 and ENSG00000142208 and 207, this GO :0000060 and protein import into nucleus, translocation and biological process this GO :0001649 and osteoblast differentiation and biological process this GO :0001893 and maternal placenta development and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0001938 and positive regulation of endothelial cell proliferation and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004712 and protein serine/threonine/tyrosine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005080 and protein kinase C binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005547 and phosphatidylinositol-3,4,5-trisphosphate binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005819 and spindle and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0005977 and glycogen metabolic process and biological process this GO :0005978 and glycogen biosynthetic process and biological process this GO :0005979 and regulation of glycogen biosynthetic process and biological process this GO :0006006 and glucose metabolic process and biological process this GO :0006412 and translation and biological process this GO :0006417 and regulation of translation and biological process this GO :0006464 and cellular protein modification process and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006469 and negative regulation of protein kinase activity and biological process this GO :0006809 and nitric oxide biosynthetic process and biological process this GO :0006915 and apoptotic process and biological process this GO :0006924 and activation-induced cell death of T cells and biological process this GO :0006954 and inflammatory response and biological process this GO :0007165 and signal transduction and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0007281 and germ cell development and biological process this GO :0007568 and aging and biological process this GO :0007596 and blood coagulation and biological process this GO :0008283 and cell proliferation and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0008637 and apoptotic mitochondrial changes and biological process this GO :0009408 and response to heat and biological process this GO :0009725 and response to hormone and biological process this GO :0010467 and gene expression and biological process this GO :0010507 and negative regulation of autophagy and biological process this GO :0010748 and negative regulation of plasma membrane long-chain fatty acid transport and biological process this GO :0010765 and positive regulation of sodium ion transport and biological process this GO :0010907 and positive regulation of glucose metabolic process and biological process this GO :0010951 and negative regulation of endopeptidase activity and biological process this GO :0010975 and regulation of neuron projection development and biological process this GO :0015630 and microtubule cytoskeleton and cellular component this GO :0015758 and glucose transport and biological process this GO :0016070 and RNA metabolic process and biological process this GO :0016071 and mRNA metabolic process and biological process this GO :0016301 and kinase activity and molecular function this GO :0016310 and phosphorylation and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0016772 and transferase activity, transferring phosphorus-containing groups and molecular function this GO :0018105 and peptidyl-serine phosphorylation and biological process this GO :0019899 and enzyme binding and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0030030 and cell projection organization and biological process this GO :0030154 and cell differentiation and biological process this GO :0030163 and protein catabolic process and biological process this GO :0030168 and platelet activation and biological process this GO :0030212 and hyaluronan metabolic process and biological process this GO :0030235 and nitric-oxide synthase regulator activity and molecular function this GO :0030307 and positive regulation of cell growth and biological process this GO :0030334 and regulation of cell migration and biological process this GO :0031018 and endocrine pancreas development and biological process this GO :0031295 and T cell costimulation and biological process this GO :0031659 and positive regulation of cyclin-dependent protein kinase activity involved in G1/S and biological process this GO :0031999 and negative regulation of fatty acid beta-oxidation and biological process this GO :0032094 and response to food and biological process this GO :0032270 and positive regulation of cellular protein metabolic process and biological process this GO :0032287 and peripheral nervous system myelin maintenance and biological process this GO :0032436 and positive regulation of proteasomal ubiquitin-dependent protein catabolic process and biological process this GO :0032869 and cellular response to insulin stimulus and biological process this GO :0032880 and regulation of protein localization and biological process this GO :0033138 and positive regulation of peptidyl-serine phosphorylation and biological process this GO :0034405 and response to fluid shear stress and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0036064 and ciliary basal body and cellular component this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0042593 and glucose homeostasis and biological process this GO :0042640 and anagen and biological process this GO :0042802 and identical protein binding and molecular function this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043154 and negative regulation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0043325 and phosphatidylinositol-3,4-bisphosphate binding and molecular function this GO :0043491 and protein kinase B signaling and biological process this GO :0043536 and positive regulation of blood vessel endothelial cell migration and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045429 and positive regulation of nitric oxide biosynthetic process and biological process this GO :0045600 and positive regulation of fat cell differentiation and biological process this GO :0045725 and positive regulation of glycogen biosynthetic process and biological process this GO :0045792 and negative regulation of cell size and biological process this GO :0045861 and negative regulation of proteolysis and biological process this GO :0045907 and positive regulation of vasoconstriction and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046209 and nitric oxide metabolic process and biological process this GO :0046326 and positive regulation of glucose import and biological process this GO :0046329 and negative regulation of JNK cascade and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0046889 and positive regulation of lipid biosynthetic process and biological process this GO :0048009 and insulin-like growth factor receptor signaling pathway and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0050999 and regulation of nitric-oxide synthase activity and biological process this GO :0051000 and positive regulation of nitric-oxide synthase activity and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0051146 and striated muscle cell differentiation and biological process this GO :0060644 and mammary gland epithelial cell differentiation and biological process this GO :0060709 and glycogen cell differentiation involved in embryonic placenta development and biological process this GO :0060716 and labyrinthine layer blood vessel development and biological process this GO :0061024 and membrane organization and biological process this GO :0070141 and response to UV-A and biological process this GO :0071260 and cellular response to mechanical stimulus and biological process this GO :0071363 and cellular response to growth factor stimulus and biological process this GO :0071364 and cellular response to epidermal growth factor stimulus and biological process this GO :0071407 and cellular response to organic cyclic compound and biological process this GO :0071456 and cellular response to hypoxia and biological process this GO :0071889 and 14-3-3 protein binding and molecular function this GO :0090004 and positive regulation of establishment of protein localization to plasma membrane and biological process this GO :0090201 and negative regulation of release of cytochrome c from mitochondria and biological process this GO :0097011 and cellular response to granulocyte macrophage colony-stimulating factor stimulus and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process this GO :0097194 and execution phase of apoptosis and biological process this GO :1900740 and positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway and biological process this GO :1901653 and cellular response to peptide and biological process this GO :1901976 and regulation of cell cycle checkpoint and biological process this GO :1902176 and negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway and biological process this GO :2001240 and negative regulation of extrinsic apoptotic signaling pathway in absence of ligand and biological process, phosphatidylinositol-3, this GO :0004672: protein kinase activity and also this GO :0004674: protein serine/threonine kinase activity and also this GO :0004712: protein serine/threonine/tyrosine kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0005080: protein kinase C binding and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0005547: phosphatidylinositol-3,4,5-trisphosphate binding and also this GO :0016301: kinase activity and also this GO :0016772: transferase activity, transferring phosphorus-containing groups and also this GO :0019899: enzyme binding and also this GO :0019901: protein kinase binding and also this GO :0030235: nitric-oxide synthase regulator activity and also this GO :0042802: identical protein binding and also this GO :0043325: phosphatidylinositol-3,4-bisphosphate binding and also this GO :0071889: 14-3-3 protein binding, this GO :0004672: protein kinase activity, this GO :0004674: protein serine/threonine kinase activity, this GO :0004712: protein serine/threonine/tyrosine kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0005080: protein kinase C binding, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0005547: phosphatidylinositol-3,4,5-trisphosphate binding, this GO :0016301: kinase activity, this GO :0016772: transferase activity, transferring phosphorus-containing groups, this GO :0019899: enzyme binding, this GO :0019901: protein kinase binding, this GO :0030235: nitric-oxide synthase regulator activity, this GO :0042802: identical protein binding, this GO :0043325: phosphatidylinositol-3,4-bisphosphate binding, this GO :0071889: 14-3-3 protein binding, nuclei, Akt1 and IDBG-174623 and ENSMUSG00000001729 and 11651, AKT1 and IDBG-635767 and ENSBTAG00000017636 and
MESH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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