AKT1 Antibody (pSer473): ATTO 390

• Catalog number
  SPC-1340D-A390
• Price
  Please ask
• Size
  100 µl

• Target
  AKT1 (pSer473)
• Conjugate
  ATTO 390
• Research Area s
  Cell Signaling, Protein Phosphorylation, Serine / Threonine Kinases, PKB / AKT, Cancer, Apoptosis, Cancer, Cancer Metabolism, Cancer, Cell Cycle, PI3k/AKT Pathway
• Alternative Name s
  RAC-PK-alpha Antibody, Protein kinase B alpha Antibody, RAC Antibody, Proto-oncogene c-Akt Antibody, Protein kinase B Antibody, RAC Alpha Antibody, AKT Antibody, EC:2.7.11.1 Antibody, AKT 1 Antibody, PRKBA Antibody, AKT1_HUMAN Antibody, AKT1 Antibody, PKB Antibody, PKB-ALPHA Antibody, MGC99656 Antibody, PKB alpha Antibody, RAC-alpha serine/threonine-protein kinase Antibody
• Category
  Antibodies
• Product Type
  Polyclonal
• Immunogen
  Synthetic phospho-peptide from human AKT1 around the phosphorylation site of serine 473 (pSer473).
• Immunogen Species
  Human
• Accession Number
  NP_001014431.1
• Gene ID
  207
• Swiss Prot
  P31749
• Applications
  WB, IHC
• Host Species
  Rabbit
• Species Reactivity
  Human, Mouse, Rat
• Antibody Dilution
  WB (1:250-1:500); IHC (1:25-1:50); optimal dilutions for assays should be determined by the user.
• Purification
  Peptide Affinity Purified
• Storage Buffer
  PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
• Specificity
  Detects endogenous levels of AKT1 only when phosphorylated at serine 473 (pSer473).
• Storage Temperature
  -20ºC
• Shipping Temperature
  Blue Ice or 4ºC
• Certificate of Analysis
  A 1:500 dilution of SPC-1340 was sufficient for detection of AKT1 (pSer473) in 10 µg of human cervical cancer cell line (hela) lysate by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.
• Cellular Localization
  Nucleus, Plasma Membrane, Cell Membrane, Cytoplasm, Cytoskeleton, Ciliary Basal Body, Microtubule, Spindle, Cytosol, Mitochondrion, Nucleoplasm, Cell-Cell Junction, Postsynapse, Protein Complex, Vesicle
• Field of Use
  For in vitro research use only.
• Technical data
• SDS
• Gross Weight kg
  1.4
• Net Weight g
  0.1
• Properties
  If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  AKT1   pSer473   ATTO   390  
• Gene symbol
  AKT1
• Short name
  AKT1 Antibody (pSer473): ATTO 390
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  IgG
• Alternative name
  v-akt murine thymoma viral oncogene homolog 1 (antibody to-) (pSer473): ATTO 390
• Alternative technique
  antibodies
• Alternative to gene target
  v-akt murine thymoma viral oncogene homolog 1, AKT and CWS6 and PKB and PKB-ALPHA and PRKBA and RAC and RAC-ALPHA, AKT1 and IDBG-22709 and ENSG00000142208 and 207, phosphatidylinositol-3, nuclei, Akt1 and IDBG-174623 and ENSMUSG00000001729 and 11651, AKT1 and IDBG-635767 and ENSBTAG00000017636 and

Gene info

• Identity
  HGNC:391
• Gene
  AKT1
• Long gene name
  AKT serine/threonine kinase 1
• Synonyms gene name
  • v-akt murine thymoma viral oncogene homolog 1
• Synonyms
  • RAC
  • PKB
  • PRKBA
  • AKT
  • RAC-alpha
• Synonyms name
  • protein kinase B
• GenBank acession
  • M63167
• Locus
  14q32.33
• Discovery year
  1986-01-01
• Entrez gene record
  207
• Pubmed identfication
  • 21214427
• RefSeq identity
  • NM_005163
• Classification
  • Pleckstrin homology domain containing
  • AKT kinases
• VEGA ID
  OTTHUMG00000170795
• Locus Specific Databases
  • LRG_721

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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