Total Aflatoxin ELISA Kit

  • Catalog number
    E4747-100
  • Price
    Please ask
  • Size
    100 assays
  • Description
    Competitive ELISA kit for the quantitative measurement of Total Aflatoxin in grain and feed samples.
  • Detection Method
    Absorbance (450 nm)
  • Species Reactivity
    Universal
  • Applications
    Sensitivity: 0.02 ppb (ng/ml), Sample recovery rate: Grain/Formula feed- 85%±15%, Peanut- 82%±15%, Edible oil- 85%±15%, Sauce/Wheat/Barley feed- 83%±15%, Beer- 84%±15%, Wine/Soy sauce/Vinegar- 87%±15%, Detection limit: Grain- 0.1 ppb; Formula feed- 0.2 ppb; Edible oil/Peanut- 0.2 ppb; Sauce/Wheat/Barley feed- 0.2 ppb; beer- 0.2 ppb; Wine/Soy sauce/Vinegar- 0.1 ppb, quantitative measurement of Total Aflatoxin in grain and feed samples., Cross-reactivity: Aflatoxin B1 (AFB1) - 100%, AflatoxinB2 (AFB2) - 80%, AflatoxinG1 (AFG1)- 75%, AflatoxinG2 (AFG2) - 45%, FlatoxinM1 (AFM1) - 8%
  • Sample Type
    Wine, Soy, sauce, vinegar, Grain, Peanut, Formula feed, Edible oil
  • Features Benefits
    Cross-reactivity: Aflatoxin B1 (AFB1) - 100%, AflatoxinB2 (AFB2) - 80%, AflatoxinG1 (AFG1)- 75%, AflatoxinG2 (AFG2) - 45%, FlatoxinM1 (AFM1) - 8%, Quantitative measurement of Total Aflatoxin in grain and feed samples., Detection limit: Grain- 0.1 ppb; Formula feed- 0.2 ppb; Edible oil/Peanut- 0.2 ppb; Sauce/Wheat/Barley feed- 0.2 ppb; beer- 0.2 ppb; Wine/Soy sauce/Vinegar- 0.1 ppb, Sensitivity: 0.02 ppb (ng/ml), Sample recovery rate: Grain/Formula feed- 85%±15%, Peanut- 82%±15%, Edible oil- 85%±15%, Sauce/Wheat/Barley feed- 83%±15%, Beer- 84%±15%, Wine/Soy sauce/Vinegar- 87%±15%
  • Storage Conditions
    4°C
  • Shipping Conditions
    Gel Pack
  • Shelf life
    12 months
  • Background
    This Total Aflatoxin ELISA Kit is based on the Competitive ELISA method. It can detect Aflatoxin (AF) in samples, such as grain, peanut, formula feed, etc. The microplate provided in this kit has been pre-coated with coupled antigen. During the reaction, AF in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-AF antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of AF. The concentration of AF in the samples can be calculated by comparing the OD of the samples to the standard curve.
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Gene target
  • Gene symbol
    AKR7A3, AKR7A2
  • Short name
    Aflatoxin ELISA Kit
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Alternative name
    Total Aflatoxin Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Tissue
    total
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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