Mouse Anti-Human Ankyrin (pan) Monoclonal IgG1, Clone S388A-60
-
Catalog numberSMC-488D-A565
-
PricePlease ask
-
Size100 µg
-
-
CloneS388A-60
-
ImmunogenFusion protein amino acids 1-1881 (full-length) of human Ankyrin-R
-
Antibody s full descriptionMouse Anti-Human Ankyrin (pan) Monoclonal IgG1 Antibody, Clone: S388A-60: ATTO 565
-
Antibody s categoryMonoclonal Antibodies
-
Antibody s other nameAnkyrin-1 Antibody, Erythrocyte Ankyrin Antibody, ANK-1 Antibody, ANK Antibody, rCG_43073 Antibody
-
Raised inMouse
-
Antibody s targetAnkyrin (pan)
-
Primary research fieldsNeuroscience, Cell Structure, Membrane Markers, Cell Signaling, Scaffolds, Cardiovascular System, Blood
-
BrandnameNone
-
Antibodies applicationsWB, IHC, ICC/IF
-
Antibody s reactivityHuman, Mouse, Rat
-
Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
-
PurityProtein G Purified
-
Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.1% sodium azide
-
Antibody s concentration1 mg/ml
-
Antibody s specificityDetects ~200kDa. Cross-reacts with Ankyrin-B and Ankyrin-G.
-
Storage recommendations-20ºC
-
Shipping recommendationsBlue Ice or 4ºC
-
Antibody certificate of analysisA 1:100 dilution of SMC-488 was sufficient for detection of Ankyrin (pan) in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
-
Antibody in cellCytoplasm , Cytoskeleton , Myofibril , Sarcomere , M line
-
Tissue specificitySee included datasheet or contact our support service
-
Scientific contextAnkyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane.
-
Bibliography1. Bennett V., Baines A.J. (2001) Physiol. Rev. 81 (3): 1353–92.
-
Released date10-Mar-2015
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
NCBI numberNP_000028.3
-
Gene number286
-
Protein numberP16157
-
Antibody s datasheetContact our support service
-
Representative figure link
-
Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin (pan) Monoclonal Antibody, Clone S388A-60 (SMC-488). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin (pan) Monoclonal Antibody (SMC-488) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Cytoskeleton. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Ankyrin (pan) Antibody (D) Composite. Western Blot analysis of Rat Brain showing detection of ~200 kDa Ankyrin-pan protein using Mouse Anti-Ankyrin-pan Monoclonal Antibody, Clone S388A-60 (SMC-488). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-Ankyrin-pan Monoclonal Antibody (SMC-488) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa. Other Band(s): ~ 80 kDa (smear). Mouse Anti-Ankyrin (pan) Antibody [S388A-60] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-488) Mouse Anti-Ankyrin-pan Antibody [S388A-60] used in Western Blot (WB) on Rat Brain (SMC-488)
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilityIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
TestStressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Latin nameMus musculus
-
Gene target
-
Gene symbolIGLV4-60, IGHVII-60-1, IGHV3-60, RCC2, OGFR, RNU6-60P, SPINK4, USP32, PPIL2, DYNC2LI1
-
Short nameMouse Anti- Ankyrin (pan) Monoclonal IgG1, Clone S388A-60
-
Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
-
Hostmouse
-
IsotypeIgG1, IgG1
-
LabelATTO 565
-
SpeciesHuman, Humans, Mouses
-
Alternative nameMouse Antibody toHuman Ankyrin (pan) monoclonal IgG1, clonality S388A-60
-
Alternative techniquemurine, antibodies
-
Clone nameS388A-60
-
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin lambda variable 4-60
-
GenBank acession
-
Locus
-
Discovery year2000-05-08
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin lambda locus at 22q11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable (II)-60-1 (pseudogene)
-
Synonyms gene name
- immunoglobulin heavy variable (II)-60-1
- immunoglobulin heavy variable (II)-60-1 pseudogene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-17
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable 3-60 (pseudogene)
-
Synonyms gene name
- immunoglobulin heavy variable 3-60
- immunoglobulin heavy variable 3-60 pseudogene
-
GenBank acession
-
Locus
-
Discovery year2000-04-04
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameregulator of chromosome condensation 2
-
Synonyms
-
Locus
-
Discovery year2005-06-07
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameopioid growth factor receptor
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2001-05-31
-
Entrez gene record
-
Pubmed identfication
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameRNA, U6 small nuclear 60, pseudogene
-
Synonyms gene
-
Synonyms gene name
- RNA, U6 small nuclear 60
-
Locus
-
Discovery year2011-08-08
-
Entrez gene record
Gene info
-
Identity
-
Gene
-
Long gene nameserine peptidase inhibitor Kazal type 4
-
Synonyms gene name
- serine protease inhibitor, Kazal type 4
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2001-09-21
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Serine peptidase inhibitors, Kazal type
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameubiquitin specific peptidase 32
-
Synonyms gene name
- ubiquitin specific protease 32
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2003-05-29
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- EF-hand domain containing
- Ubiquitin specific peptidases
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namepeptidylprolyl isomerase like 2
-
Synonyms gene name
- peptidylprolyl isomerase (cyclophilin)-like 2
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year1999-10-29
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Spliceosomal Bact complex
- U-box domain containing
- Cyclophilin peptidylprolyl isomerases
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namedynein cytoplasmic 2 light intermediate chain 1
-
Synonyms
-
Locus
-
Discovery year2005-11-29
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Dynein 2 complex subunits
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data