Syrian Hamster Anti Mouse CD28 Antibody-FITC

• Catalog number
  MO28F(V25)
• Price
  Please ask
• Size
  0,025 mg

• Description
  Syrian Hamster Anti Mouse CD28 Antibody-FITC is a mouse monoclonal antibody, specific for [37.51] clones, this reagent was conjugated with FITC format and reported for use in flow cytometric analysis to identify CD28 glycoproteins in thymocytes, peripheral T cells, NK cells, CD28 plays a role of T and Nk cells costimulation, activation and proliferation, this antibody acts as a stimulant of cytoxin production, T cell proliferation and CTL development .
• Other rodent antibodies
• Clone
  37.51
• Conjugate
  FITC
• Gene Name
  CD28
• Other Names of gene
  Tp44, T44
• Concentration
  500 µg/mL
• Application
  Flow Cytometry Method
• Immunogen
  Mouse EL-4 (T-cell lymph oma) Cells
• Formulation
  Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
• Storage
  2-8°C, store in dark, do not freeze
• Expiration
  After 1 year
• Remark
  For Recherche Use Only.
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
• Properties
  If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• French translation
  anticorps

• Gene target
  Syrian   CD28  
• Gene symbol
  CD28
• Short name
  Syrian Anti Mouse CD28 Antibody-FITC
• Technique
  anti mouse, Antibody, Mouse, anti, FITC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, Fluorescein, mouses
• Host
  Hamster
• Isotype
  Syrian Hamster IgG2
• Label
  FITC
• Species
  Mouse, Hamsters, Mouses
• Alternative name
  Syrian Hamster CD28 Mouse Monoclonal Antibody [37.51]-FITC
• Alternative technique
  antibodies, murine, fluorescine
• Alternative to gene target
  CD28 molecule, Tp44, CD28 and IDBG-79183 and ENSG00000178562 and 940, identical protein binding, Cell surfaces, Cd28 and IDBG-159608 and ENSMUSG00000026012 and 12487, CD28 and IDBG-643371 and ENSBTAG00000007445 and 281050

Gene info

• Identity
  HGNC:1653
• Gene
  CD28
• Long gene name
  CD28 molecule
• Synonyms gene name
  • CD28 antigen (Tp44)
• Synonyms name
  • T-cell-specific surface glycoprotein
• GenBank acession
  • J02988
• Locus
  2q33.2
• Discovery year
  1988-05-11
• Entrez gene record
  940
• Pubmed identfication
  • 1355979
• RefSeq identity
  • NM_006139
• Classification
  • CD molecules
  • Ig-like cell adhesion molecule family
  • V-set domain containing
• VEGA ID
  OTTHUMG00000132878

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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