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Clone
BB70
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Immunogen
Chicken HSP70/HSP90 complex
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Antibody s full description
Mouse Anti-Chicken HSP70/HSC70 Monoclonal IgG2a Antibody, Clone: BB70: PE/ATTO 594
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
HSC54 Antibody, HSC70 Antibody, HSC71 Antibody, HSP70 1 Antibody, HSP701/HSP70 2 Antibody, HSP70.1 Antibody, HSP71 Antibody, HSP72 Antibody, HSP73 Antibody, HSPA1 Antibody, HSPA10 Antibody, HSPA1A Antibody, HSPA1B Antibody, LAP1 Antibody, NIP71 Antibody
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Raised in
Mouse
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Antibody s target
HSP70/HSC70
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Primary research fields
Cancer, Heat Shock
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Brandname
None
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Antibodies applications
WB, IHC, IP
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Antibody s reactivity
Human, Mouse, Rat, Bovine, Sheep, Dog, Beluga, Fish, Guinea Pig (Cavia porcellus), Pig, Hamster, Rabbit, Chicken, African clawed frog (Xenopus laevis), Fruit Fly (Drosophila melanogaster), Yeast
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Antibody s dilutions
WB (1:1000), IHC (1:200), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.2, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~72 (HSP) and ~73kDa (HSC).
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-106 was sufficient for detection of HSP70 and HSC70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at more.
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Bibliography
1. Zho J. (1998) Cell 94 : 471-480. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1): 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nat Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Smith D.F., et al, (1993) Mol. Cell. Biol. 13(2): 869-876. 8. Prapapanich V., et al. (1996) Mol. Cell. Biol. 16(11):6200-6207. 9. Fernandez-Funez et al., (2000) Nature 408(6808): 101-106.
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Released date
1-Jun-2007
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PubMed number
24934503, 24548207, 24548239, 24239008
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Tested applications
Western Blot, Western Blot, Western Blot, Protein Binding Assay
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Tested reactivity
Human, Human, Human, Human
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NCBI number
NP_001006686.1
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Gene number
423504
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Protein number
P08106
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (SMC-106). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-106) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (SMC-106). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-106) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Western Blot analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (SMC-106). Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-106) at 1:1000. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (SMC-106). Tissue: hepatocyte nuclei. Species: Rat. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-106) at 1:200. Courtesy of: G. Matic, University of Belgrade, Serbia. Western Blot analysis of Human HeLa cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (SMC-106). Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-106) at 1:1000. Secondary Antibody: HRP Goat Anti-Rat. Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (SMC-106). Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-106) at 1:200. Courtesy of: G. Matic, University of Belgrade, Serbia. Mouse Anti-Hsp70 Antibody [BB70] used in Immunohistochemistry (IHC) on Mouse inflamed colon (SMC-106) Mouse Anti-Hsp70 Antibody [BB70] used in Immunohistochemistry (IHC) on Human colon carcinoma (SMC-106) Mouse Anti-Hsp70 Antibody [BB70] used in Western Blot (WB) on Bovine MDBK cell lysates (SMC-106) Mouse Anti-Hsp70 Antibody [BB70] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Rat hepatocyte nuclei (SMC-106) Mouse Anti-Hsp70 Antibody [BB70] used in Western Blot (WB) on Human HeLa cell lysates (SMC-106) Mouse Anti-Hsp70 Antibody [BB70] used in Immunohistochemistry (IHC) on Rat hepatocytes (SMC-106)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.2
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
