Donkey anti Chicken IgY H + L biotin

  • Catalog number
    43R-ID053bt
  • Price
    Please ask
  • Size
    500 µl
  • Category
    Secondary Antibody
  • Antibody Subtype
    Conjugated Secondary Antibodies
  • Area of research
    Immunology
  • Type of Immunogen
    Donkey anti-chicken IgY (H + L) (biotin) was raised in donkey using chicken IgG (H & L) ..
  • Raised in
    Donkey
  • Specificity
    Chicken
  • Cross Reactivity
    Should be determined by the end user
  • Conjugate Tag
    Biotin
  • Method of Purification
    Donkey anti Chicken IgY (H + L) (biotin) was purified by affinity chromatography.
  • Form Buffer
    Lyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA and 0.05% NaN3 as preservative.
  • Storage
    Store at 4 deg C.
  • Shipping conditions
    Blue Ice
  • Tested for
    ELISA; FC; ICC; IHC; WB
  • Usage Recommendations
    ELISA: 1:20,000-1:400,000, FC: 1:200-1:1,000, ICC: 1:500-1:5,000, IHC: 1:500-1:5,000, WB: 1:20,000-1:400,000
  • URL
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. Chicken yolk polyclonal Immunoglobulin Y (abbreviated as IgY) is a type of immunoglobulin which is the major antibody in bird, reptile, and lungfish blood. It is also found in high concentrations in chicken egg yolk. As with the other immunoglobulins, IgY is a class of proteins which are formed by the immune system in reaction to certain foreign substances, and specifically recognize them.
  • Conjugation
    Biotinylated
  • Latin name
    Equus africanus asinus
  • Gene target
    -
  • Gene symbol
    CALD1, FUT1, L3MBTL2
  • Short name
    anti IgY H + L
  • Technique
    anti, IgY, antibody to, IgYs
  • Host
    Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
  • Isotype
    IgY antibody
  • Label
    Biotin
  • Species
    chicken, Chickens, Donkeys
  • Alternative name
    Donkey anti Chicken IgY (H + L) secondary antibody (biotin)
  • Alternative technique
    antibodies, bird
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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