Blasticidin solution, 10mg/ml in 20mM HEPES

  • Catalog number
    B006-20 ml
  • Price
    Please ask
  • Size
    20ml
  • Chemical formula
    C17H26N8O5*HCl
  • CAS number
    3513-03-9
  • Transportation conditions
    dry ice
  • Storage Temp
    Upon receipt place and keep the vial frozen at -20 degrees Celcius.
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Description
    The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.
  • Gene target
    Blasticidin   10mg   20mM  
  • Short name
    Blasticidin , 10mg/ in 20mM
  • Alternative name
    Blasticidin solution, 10mg/milliliter in 20mM HEPES
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