Rat CD34 Mouse Monoclonal Antibody [MEC14.7]-Biotin
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Catalog number
BD-MO34B(V100)
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Price
Please ask
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Size
0,1 mg
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Description
Rat CD34 Mouse Monoclonal Antibody [MEC14.7]-Biotin is a mouse monoclonal antibody, specific for [MEC14.7] clones, this product was conjugated with Biotin format and reprted for use in flow cytometric analysis to react with CD34 antigens in hematopoietic progenitors, brain, testis, and also in endothelial cells, the role of CD34 is possibly adhesion of stem cells to extracellular matrix or stromal cells.
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Clone
MEC14.7
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Conjugate
Biotin
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Gene Name
CD34
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Other Names of gene
Mucosialin
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Concentration
500 µg/mL
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Application
Flow Cytometry Method
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Immunogen
Cells transfected with mouse CD40
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Formulation
Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
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Storage
2-8°C, store in dark, do not freeze
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Expiration
After 1 year
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Remark
For Recherche Use Only.
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Properties
If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Stem cell or hematopoietic marker. The most used affinity clone is QBEND10. We compare the affinity to this clone.
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Conjugation
Biotinylated
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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French translation
anticorps
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Gene target
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Gene symbol
CD34
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Short name
CD34 Mouse Monoclonal Antibody [MEC14 7]-
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Technique
Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
Rat, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
Rat IgG2a
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Label
Biotin
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Species
Mouse, Mouses, Rats
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Alternative name
Rat Anti Mouse CD34 Antibody-Biotin
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Alternative technique
antibodies, murine
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Alternative to gene target
CD34 molecule, CD34 and IDBG-106399 and ENSG00000174059 and 947, sulfate binding, Extracellular, Cd34 and IDBG-209317 and ENSMUSG00000016494 and 12490, CD34 and IDBG-638449 and ENSBTAG00000010562 and 281051
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Gene info
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Identity
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Gene
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Long gene name
CD34 molecule
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Synonyms gene name
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GenBank acession
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Locus
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Discovery year
1990-09-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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