Rat CD127 Mouse Monoclonal Antibody [A7R34]-FITC

• Catalog number
  BD-MO127F(V25)
• Price
  Please ask
• Size
  0,025 mg

• Description
  Rat CD127 Mouse Monoclonal Antibody [A7R34]-FITC is a mouse monoclonal antibody, specific for [A7R34] clones, this product was conjugated with FITC format and reported for use in flow cytometric analysis to interact with CD127 glycoproteins that found on bone marrow stem cells, thymocytes, the early stage of pre-B cells, and on peripheral T cells, CD127 plays a role of proliferation and development.
• Other antibodies
• Clone
  A7R34
• Conjugate
  FITC
• Gene Name
  CD127
• Other Names of gene
  IL-7 receptor α chain, IL-7Rα
• Concentration
  500 µg/mL
• Application
  Flow Cytometry Method
• Immunogen
  IL-7Ra-IgG1 fusion protein
• Formulation
  Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
• Storage
  2-8°C, store in dark, do not freeze
• Expiration
  After 1 year
• Remark
  For Recherche Use Only.
• Properties
  If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus
• French translation
  anticorps

• Gene target
  CD127   Monoclonal   [A7R34]  
• Gene symbol
  IL7R
• Short name
  CD127 Mouse Monoclonal Antibody [A7R34]-FITC
• Technique
  Mouse monoclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
• Host
  Rat, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
• Isotype
  Rat IgG2a
• Label
  FITC
• Species
  Mouse, Mouses, Rats
• Alternative name
  Rat Anti Mouse CD127 Antibody-FITC
• Alternative technique
  antibodies, murine, fluorescine

Gene info

• Identity
  HGNC:6024
• Gene
  IL7R
• Long gene name
  interleukin 7 receptor
• Synonyms
  • CD127
  • IL7RA
• GenBank acession
  • M29696
• Locus
  5p13.2
• Discovery year
  1991-08-07
• Entrez gene record
  3575
• Pubmed identfication
  • 2317865
• Classification
  • Interleukin receptors
  • Fibronectin type III domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000090791
• Locus Specific Databases
  • IL7Rbase: Mutation registry for Interleukin-7 receptor α deficiency
  • LRG_74

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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