Mouse IgG3 Human Isotype Control Monoclonal Antibody [PPV-07]-FITC

• Catalog number
  BD-ICIGG3F-100
• Price
  Please ask
• Size
  0,1 mg

• Description
  Mouse IgG3 Human Isotype Control Monoclonal Antibody [PPV-07]-FITC is a mouse IgG3 monoclonal antibody type human isotype control, produced by [PPV-07] clones, this reagent was conjugated with FITC format and reported for use in flow cytometric analysis as a negative control, it's used for testing the reliabilty of biological tests. Use the same concetration of the specific antibody of interest.
• Other human isotypes
• Clone
  PPV-07
• Conjugate
  FITC
• Application
  Flow Cytometry Method
• Formulation
  Phosphate buffred solution, 0,09% sodium Azide, may contain carrier protein-stabilizer
• Expiration
  After 1 year
• Storage
  2-8°C, store in dark, do not freeze
• Remark
  For Recherche Use Only.
• Properties
  If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
• Additional description
  Isotype or positive controls by peptides, antibodies and deactivated samples.
• Conjugation
  Anti-FITC Antibody
• Group
  isotypes
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• French translation
  anticorps

• Gene target
  IgG3   Monoclonal   [PPV-07]  
• Gene symbol
  NMD3, HCG4P7
• Short name
  Mouse IgG3 Isotype Control Monoclonal Antibody [PPV-07]-FITC
• Technique
  Antibody, Control, Isotype, Mouse, FITC, antibodies against human proteins, antibodies for, controls, Fluorescein, Isotypes and controls, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG3, IgG3
• Label
  FITC
• Species
  Human, Humans, Mouses
• Alternative name
  Anti Mouse IgG3 Human Isotype Control-FITC
• Alternative technique
  antibodies, control, controls, murine, fluorescine
• Tissue
  control

Gene info

• Identity
  HGNC:24250
• Gene
  NMD3
• Long gene name
  NMD3 ribosome export adaptor
• Synonyms gene name
  • NMD3 homolog (S. cerevisiae)
• Synonyms
  • CGI-07
• GenBank acession
  • BC013317
• Locus
  3q26.1
• Discovery year
  2005-01-26
• Entrez gene record
  51068
• Pubmed identfication
  • 10810093
  • 23782956
  • 12773398
• RefSeq identity
  • NM_015938
• VEGA ID
  OTTHUMG00000159063

Gene info

• Identity
  HGNC:22926
• Gene
  HCG4P7
• Long gene name
  HLA complex group 4 pseudogene 7
• Synonyms
  • HCGIV-07
  • HCGIV.C
  • HCGIV-7
• Synonyms name
  • HCGIV-07 pseudogene
• Locus
  6p21.3
• Discovery year
  2003-09-01
• Entrez gene record
  353004
• Pubmed identfication
  • 10557312
• RefSeq identity
  • NG_016116
• VEGA ID
  OTTHUMG00000130509

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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