Mouse CD45.1 Mouse Monoclonal Antibody [A20]-FITC
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Catalog numberBD-MO451F(V25)
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Price:
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Size0,025 mg
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DescriptionMouse CD45.1 Mouse Monoclonal Antibody [A20]-FITC is a mouse monoclonal antibody, specific for [A20] clones, this reagent was conjugated with FITC format and reported for use in flow cytometric analysis to identify CD45.1 antigens in all hematopoietic cells but not in mature erythrocytes and platelets, CD45.1 plays a role of T and B cells activation, phosphatase and signal transduction.
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CloneA20
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ConjugateFITC
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Gene NameCD45.1
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Other Names of geneT200, Ly-5.1, LCA
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Concentration500 µg/mL
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ApplicationFlow Cytometry Method
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ImmunogenSJL mouse thymocytes and splenocytes
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FormulationAqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
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Storage2-8°C, store in dark, do not freeze
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ExpirationAfter 1 year
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RemarkFor Recherche Use Only.
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PropertiesIf you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolTNFAIP3, IGKV1-27, PTPRCAP, PTPRC
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Short nameMouse CD45 1 Mouse Monoclonal Antibody [A20]-FITC
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TechniqueMouse monoclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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IsotypeMouse IgG2a
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LabelFITC
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SpeciesMouse, Mouses
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Alternative nameMouse Anti Mouse CD45.1 Antibody-FITC
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Alternative techniqueantibodies, murine, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameTNF alpha induced protein 3
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Synonyms gene name
- tumor necrosis factor, alpha-induced protein 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-10-20
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Entrez gene record
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Pubmed identfication
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Classification
- OTU domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1-27
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type C associated protein
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Synonyms
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Locus
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Discovery year1996-04-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type C
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
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Tree numbers
- E01.370.225.500.195.107.595.500.150
- E01.370.225.625.107.595.500.150
- E05.200.500.195.107.595.500.150
- E05.200.625.107.595.500.150
- E05.242.195.107.595.500.150
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data