FITC-Conjugated Rabbit Polyclonal IgG Isotype Control

  • Catalog number
    RBPLF-100T
  • Price
    Please ask
  • Size
    100 test
  • Clone
    POLYCLONAL
  • Conjugated
    FITC
  • Tested application
    Flow Cytometry
  • Long description
    This FITC-Conjugated Rabbit Polyclonal IgG Isotype Control was affinity purified from rabbit serum. It does not cross-react with any human or animal antigen
  • Storage buffer
    PBS, 0,09% sodium Azide, may contain carrier protein-stabilizer
  • Shipping conditions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Technical datasheet
    Please contact us to receive the latest datasheet, the certificate of analysis or the MSDS
  • Regulation status
    This antibody is intended for Research Use Only.
  • Description
    Isotype or positive controls by peptides, antibodies and deactivated samples.
  • Properties
    This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
  • Conjugation
    Anti-FITC Antibody
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by Immunostep. Rabbit antibodies are very stable and can be stored for several days at room temperature. Immunostep adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Group
    isotypes, Polyclonals and antibodies
  • Latin name
    Oryctolagus cuniculus
  • Gene target
  • Short name
    FITC-Conjugated Rabbit Polyclonal IgG Isotype Control
  • Technique
    Polyclonal, Control, Isotype, Rabbit, FITC, IgG, antibody Conjugates, controls, Fluorescein, IgGs, Isotypes and controls, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    IgG, IgG
  • Label
    FITC
  • Alternative name
    fluorecein-coupled production species: rabbit polyclonal Immunoglobulin G isoform reference
  • Alternative technique
    polyclonals, control, controls, rabbit-anti, fluorescine, immunoglobulins
  • Tissue
    control
MeSH Data
  • Name
  • Concept
    Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
  • Tree numbers
    • E01.370.225.500.386
    • E05.196.712.516.600.240
    • E05.200.500.386
    • E05.242.386
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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