ExoComplete TM - Reverse Transcription Reagent
-
Exosome isolation kit purification content
RT Buffer A (1 x 1,700 µL ), RT Enzyme Mix B (1 x 30 µL), Aluminum seal (1)
-
-
Publications with ExoComplete
unctional Analysis of Exosomes Derived from EBV-Infected Cells. Isolation and Characterization of Exosomes Released by EBV-Immortalized Cells. Plasma exosomes are enriched in Hsp70 and modulated by stress and cortisol in rainbow trout. Organelles in health and diseases. Identification of urinary exosomal non-coding RNAs as novel biomarkers in Chronic Kidney Disease. Primary Human and Rat Beta Cells Release the Intracellular Autoantigens GAD65, IA-2 and Proinsulin in Exosomes Together with Cytokine-Induced Enhancers of Immunity. Experimental, Systems and Computational Approaches to Understanding the MicroRNA-Mediated Reparative Potential of Cardiac Progenitor Cell-Derived Exosomes From Pediatric Patients. MSC exosome as a cell-free MSC therapy for cartilage regeneration: implications for osteoarthritis treatment.
-
Exocomplete s data sheet
Functional Analysis of Exosomes Derived from EBV-Infected Cells. Isolation and Characterization of Exosomes Released by EBV-Immortalized Cells. Plasma exosomes are enriched in Hsp70 and modulated by stress and cortisol in rainbow trout. Organelles in health and diseases. Identification of urinary exosomal non-coding RNAs as novel biomarkers in Chronic Kidney Disease. Primary Human and Rat Beta Cells Release the Intracellular Autoantigens GAD65, IA-2 and Proinsulin in Exosomes Together with Cytokine-Induced Enhancers of Immunity. Experimental, Systems and Computational Approaches to Understanding the MicroRNA-Mediated Reparative Potential of Cardiac Progenitor Cell-Derived Exosomes From Pediatric Patients. MSC exosome as a cell-free MSC therapy for cartilage regeneration: implications for osteoarthritis treatment.
-
-
-
Description
Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs. The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
-
Goup
reverse transcription
MeSH Data
-
Name
-
Concept
Scope note:
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
-
Tree numbers
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products
