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Description
Fetal Bovine Serum Mesenchymal Stem Cell Tested USDA Approved, Sterile filtered, is an amber liquid. It is a mixture of growth factors, proteins, vitamins, trace elements, carbohydrates, lipids and hormones That is used as an universal growth supplement effective for most types of human and animal (including insect) cells.
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Packing
100ML
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Shelf life
48 months.
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Storage
Store at -10°C to -40°C away from bright light.
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Appearance
Amber liquid
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PH
6.8 - 8.2
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Osmolality
280 – 340 mOsm/KgH2 O
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Endotoxin
Value EU/ml
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Hemoglobin
< 20mg/dl
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Identity
Typical
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Protein
Total protein : 3.0 - 4.5 g/dl Albumin : value g/dl α-Globulin : value g/dl β-Globulin : value g/dl γ-Globulin : value g/dl IgG : < 250μg/ml
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Sterility Testing
Aerobic bacteria : Not detected Anaerobic bacteria : Not detected Fungi : Not detected Mycoplasma : Not detected
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Virus testing
Bovine Virus Diarrhea Virus : Not detected (BVD-V) Bovine Herpes Virus 1 (BHV-1) : Not detected Parainfluenza Type 3 (PI-3) : Not detected
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Antibody testing
BVD-1 Antibody titer : Value BVD-2 Antibody titer : Value
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Growth promotion and cytotoxicity
Each lot of serum is tested for growth promotion and cytotoxicity. Growth promotion ability of serum is assessed by supplementing medium with 10% serum and analyzing the cell lines qualitatively for morphology and qualitatively by estimating cell counts.
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Uses
Laboratory chemicals, Manufacture of substances
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Personal Protection
Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
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Toxicological Information
NA
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Ordering
To order Fetal Bovine Serum Mesenchymal Stem Cell Tested USDA Approved, Sterile filtered, please use the Cat. Nr.RM10845-1X100ML and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Fetal Bovine Serum Mesenchymal Stem Cell Tested USDA Approved, Sterile filtered
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Additional description
For cells, cell lines and tissues in culture till half confluency. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request. This 1 has passed a sterilization (or sterilization) by filtration or inactivation that has eliminated (removed) or killed (deactivated) all forms of DNA, RNA and enzymes. Also life and other biological agents (such as viruses which some do not consider to be alive but are biological pathogens nonetheless), excluding prions which cannot be killed, including transmissible agents (such as fungi, bacteria, viruses, prions, spore forms, unicellular eukaryotic organisms such as Plasmodium, etc.) present in a specified reagent or on a surface, a volume of fluid, or in a compound such as biological culture medias filtered. Sterilization was achieved with one or more of the following heat, chemicals, irradiation, high pressure, and filtration. Sterilization is distinct from disinfection, sanitization, and pasteurization in that sterilization kills, deactivates, or eliminates all forms of life and other biological agents.
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Group
Sera
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About
Serum should be stored at +5°C
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Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
