RAT IgG2a NEGATIVE CONTROL:FITC conjugated

Old catalog number

26-783-315892
Storage temperature

store cold
Expiry date

1 y
Category

research antibodies, ELISAs, recombinant proteins, vectors and reagents for laboratory use
Description

Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.
Properties

This genways Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. genways FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody
About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name

Rattus norvegicus

Gene target

IgG2a NEGATIVE
Short name

IgG2a NEGATIVE CONTROL:FITC conjugated
Technique

Negative, Control, FITC, antibody Conjugates, controls, Fluorescein, negatif
Host

Rat
Isotype

IgG2a
Label

FITC
Species

Rat, Rats
Alternative name

RAT IgG2a NEGATIVE reference:fluorecein coupled
Alternative technique

controls, fluorescine
Tissue

control

Name

Negative Staining
Concept

Scope note: The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
Tree numbers
- E01.370.225.500.620.670.520
- E01.370.225.750.600.670.520
- E05.200.500.620.670.520
- E05.200.750.600.670.520
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data