Immunoglobulin G (IgG), Rabbit, F(ab')2 -biotin conjugated

Old catalog number

10-251-409187
Storage temperature

store cold
Expiry date

1 y
Category

research antibodies, ELISAs, recombinant proteins, vectors and reagents for laboratory use
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
Group

f(ab')2s
About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by genways. Rabbit antibodies are very stable and can be stored for several days at room temperature. genways adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name

Oryctolagus cuniculus

Gene target

Immunoglobulin IgG Fab
Short name

Immunoglobulin G (IgG), Rabbit, F(ab')2 - conjugated
Technique

F(ab')2, Rabbit, IgG, antibody Conjugates, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. IgGs
Host

Rabbit, Rabbits
Isotype

IgG, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, IgG
Label

Biotin
Alternative name

Immunoglobulin G (Immunoglobulin G), production species: rabbit, F(Antibody')2 -biotinilated coupled
Alternative technique

secondairy, rabbit-anti, immunoglobulins