amfiRivert Reverse Transcriptase Reaction Buffer(5X)

• Catalog number
  A1203-001
• Price
  Please ask
• Size
  6x1ml

• Description
  Buffering solutions are useful to keep the pH range sable when using this reagent of genDEPOT. Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
• Goup
  reverse transcription

• Gene target
  amfiRivert   Transcriptase  
• Short name
  amfiRivert Reverse Transcriptase (5X)
• Technique
  reverse
• Alternative name
  amfiRivert Reverse Transcriptase Reaction solution(5X)
• Alternative technique
  transctription

MeSH Data

• Name
  Reverse Transcriptase Polymerase Chain Reaction
• Concept
  Scope note: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
• Tree numbers
  • E05.393.620.500.725
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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