Streptavidin protein Cy3.5
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Catalog number
65C-CE0305
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Price
Please ask
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Size
1 mg
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Category
Proteins
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Antibody Subtype
Conjugated Proteins
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Area of research
Miscellaneous
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Reactivity
Streptomyces avidinii
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Conjugate Tag
CY3.5
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Type of protein
Binding Protein
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Method of Purification
Streptavidin protein (Cy3.5) was purified by Protein A affinity chromatography.
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Form Buffer
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
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Storage
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
FC; IF
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Usage Recommendations
DC: 1:200-1:400, IF: 1:100-1:1,000
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Properties
Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Streptavidin protein .5 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This fitzgerald antibody is well suited for fluorescent microscopy.
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Conjugation
cy3 conjugation kit
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