SIV mac251 gp120 antibody FITC
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Catalog number
60-00112-3
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Price
Please ask
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Size
50 µg
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Category
Primary Antibody
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Antibody Subtype
Conjugated Polyclonal Antibodies
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Area of research
Infectious Disease
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Type of Immunogen
SIV mac251 gp120 antibodies were raised in rabbit using full length recombinant gp120 (SIV-1mac251) produced in Baculovirus Expression System
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Raised in
Rabbit
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Specificity
SIV mac251 gp120
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Conjugate Tag
FITC
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Concentration
50 ug/vial
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Form Buffer
Provided liquid as a purified IgG in PBS buffer, the FITC label is attached to the free amine group, no preservatives added.
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Storage
Ships on dry ice. Upon receipt store at -70 deg C or lower. Avoid repeated freeze/thaw cycles
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Shipping conditions
Dry Ice
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Tested for
ELISA; WB
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Usage Recommendations
ELISA: 1:500-1:1000, WB: 1:500-1:1000
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Assay Information
Binds to recombinant gp120 (SIVmac251)
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Short name
SIV mac251 gp120 antibody FITC
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Label
FITC
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Alternative name
Rabbit polyclonal SIV mac251 gp120 antibody (FITC)
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Alternative technique
antibodies, fluorescine
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Virus
siv
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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