Sheep anti Pig IgG Alk Phos

Category

Secondary Antibody
Antibody Subtype

Conjugated Secondary Antibodies
Area of research

Immunology
Raised in

Sheep
Specificity

Pig
Cross Reactivity

Should be determined by the end user
Isotypes

IgG
Conjugate Tag

Alkaline Phosphatase
Method of Purification

Sheep anti Pig IgG (Alk Phos) was purified by affinity chromatography.
Concentration

0.5 mg/ml
Form Buffer

50 mM HEPES pH 7.1, 0.1M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 with 0.2% BSA and 0.09% NaN3.
Storage

Store at 4 deg C.
Shipping conditions

Blue Ice
Tested for

ELISA; ICC; IHC; WB
Usage Recommendations

ELISA: 1:200-1:2,500, ICC: 1:100-1:250, IHC: 1:100-1:250, WB: 1:100-1:500
URL
More info
Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Pigs and the smaller guinea pigs are frequent used as models for humans.

Gene target

Alk Phos
Short name

anti IgG Alk Phos
Technique

anti, IgG, antibody to, IgGs
Host

Sheep
Isotype

IgG, IgG
Species

Pig, Pigs and Guinea pigs, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
Alternative name

Sheep anti Pig IgG secondary antibody (Alk Phos)
Alternative technique

antibodies, immunoglobulins
Alternative to gene target

anaplastic lymphoma receptor tyrosine kinase, CD246 and NBLST3, ALK and IDBG-42084 and ENSG00000171094 and 238, transferase activity, Plasma membranes, Alk and IDBG-197259 and ENSMUSG00000055471 and 11682, ALK and IDBG-636042 and ENSBTAG00000007379 and 536642

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data