Sheep anti Bovine IgM HRP

Category

Secondary Antibody
Antibody Subtype

Conjugated Secondary Antibodies
Area of research

Immunology
Raised in

Sheep
Specificity

Bovine
Cross Reactivity

Should be determined by the end user
Isotypes

IgG
Conjugate Tag

HRP
Method of Purification

Sheep anti Bovine IgM (HRP) was purified by affinity chromatography.
Concentration

1.0 mg/ml
Form Buffer

PBS with 0.2% BSA, and 0.1% Pro-Clean 400.
Storage

Store at 4 deg C.
Shipping conditions

Blue Ice
Tested for

ELISA; ICC; IHC; WB
Usage Recommendations

ELISA: 1:10,000-1:100,000, ICC: 1:200-1:500, IHC: 1:200-1:500, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000
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More info
Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties

HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
Conjugation

HRP Conjugated

Short name

anti IgM
Technique

anti, IgM, antibody to, IgMs
Host

Sheep
Isotype

IgM, IgM
Label

HRP
Species

Bovine, Bos Taurus genes are available form the Bovine Genome database. The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
Alternative name

Sheep anti Bovine IgM secondary antibody (HRP)
Alternative technique

antibodies, immunoglobulin-m

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data