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Category
Secondary Antibody
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Antibody Subtype
Purified Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Rabbit anti-human IgM was raised in rabbit using human IgM (Fc5m) fragment ..
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Raised in
Rabbit
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Specificity
Human IgM
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Cross Reactivity
Should be determined by the end user
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Method of Purification
Rabbit anti Human IgM was purified by affinity chromatography.
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Concentration
Batch dependent - please inquire should you have specific requirements
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Form Buffer
0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.
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Storage
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
ELISA; IHC; WB
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Usage Recommendations
ELISA: 1:40,000-1:160,000, IHC: 1:500-1:3,000, WB: 1:500-1:3,000
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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About
Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus