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Category
Secondary Antibody
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Antibody Subtype
Purified Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Rabbit anti-chicken IgG was raised in rabbit using chicken IgG F(ab')2 fragment ..
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Raised in
Rabbit
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Specificity
Chicken IgG
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Cross Reactivity
Should be determined by the end user
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Method of Purification
Rabbit anti Chicken IgG was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
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Concentration
5.0 mg/ml
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Form Buffer
0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.
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Storage
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
ELISA; IHC; WB
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Usage Recommendations
ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus