PPIL2 protein His tag

• Catalog number
  80R-1269
• Price
  Please ask
• Size
  50 µg

• Category
  Proteins
• Antibody Subtype
  Conjugated Proteins
• Area of research
  Protein Modification & Stress Response
• Reactivity
  Human
• Residues
  1-527 amino acids: MGSSHHHHHH SSGLVPRGSH MGKRQHQKDK MYITCAEYTH FYGGKKPDLP QTNFRRLPFD HCSLSLQPFV YPVCTPDGIV FDLLNIVPWL KKYGTNPSNG EKLDGRSLIK LNFSKNSEGK YHCPVLFTVF TNNTHIVAVR TTGNVYAYEA VEQLNIKAKN FRDLLTDEPF SRQDIITLQD PTNLDKFNVS NFYHVKNNMK IIDPDEEKAK QDPSYYLKNT NAETRETLQE LYKEFKGDEI LAATMKAPEK KKVDKLNAAH YSTGKVSASF TSTAMVPETT HEAAAIDEDV LRYQFVKKKG YVRLHTNKGD LNLELHCDLT PKTCENFIRL CKKHYYDGTI FHRSIRNFVI QGGDPTGTGT GGESYWGKPF KDEFRPNLSH TGRGILSMAN SGPNSNRSQF FITFRSCAYL DKKHTIFGRV VGGFDVLTAM ENVESDPKTD RPKEEIRIDA TTVFVDPYEE ADAQIAQERK TQLKVAPETK VKSSQPQAGS QGPQTFRQGV GKYINPAATE QQRKSPQPVP LSPCPRRSPV GVLGTSAPGS SRLPDDH
• Conjugate Tag
  His tag
• Type of protein
  Recombinant
• Expression System
  Escherichia coli
• Grade Purity
  Grater than 95% pure
• Concentration
  0.5 mg/ml (lot specific)
• Form Buffer
  Provided as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 20% glycerol and 0.1M NaCl.
• Storage
  Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Shipping conditions
  Blue Ice
• Tested for
  SDS-PAGE
• Bioactivity
  Specific activity is > 300 nM/min/ug, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 DEG C in Tris-Hcl pH8.0 using chymotrypsin.
• Assay Information
  1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant PPIL2 protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes
• Additional Information
  PPIL2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
• URL
• More info
• Conjugation
  histidine

• Gene target
  PPIL2   protein   His   tag  
• Gene symbol
  PPIL2
• Short name
  PPIL2 protein His tag
• Technique
  Tags
• Label
  His
• Alternative name
  Purified recombinant Human PPIL2 protein
• Alternative to gene target
  peptidylprolyl isomerase (cyclophilin)-like 2, CYC4 and Cyp-60 and CYP60 and hCyP-60 and UBOX7, PPIL2 and IDBG-2106 and ENSG00000100023 and 23759, ubiquitin-ubiquitin ligase activity, nuclei, Ppil2 and IDBG-137979 and ENSMUSG00000022771 and 66053, PPIL2 and IDBG-637559 and ENSBTAG00000021121 and 511467

Gene info

• Identity
  HGNC:9261
• Gene
  PPIL2
• Long gene name
  peptidylprolyl isomerase like 2
• Synonyms gene name
  • peptidylprolyl isomerase (cyclophilin)-like 2
• Synonyms
  • UBOX7
  • CYC4
  • Cyp-60
• Synonyms name
  • U-box domain containing 7
  • peptidyl-prolyl cis-trans isomerase-like 2
• Locus
  22q11.21
• Discovery year
  1999-10-29
• Entrez gene record
  23759
• Pubmed identfication
  • 10591208
• Classification
  • Spliceosomal Bact complex
  • U-box domain containing
  • Cyclophilin peptidylprolyl isomerases
• VEGA ID
  OTTHUMG00000191763

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