Parvovirus IgM ELISA kit

• Catalog number
  55R-IB79807
• Price
  Please ask
• Size
  96 wells

• Category
  Research kit
• Area of research
  Infectious Disease
• Storage
  Store at 2-8 deg C
• Shipping conditions
  Blue Ice
• Tested for
  ELISA
• Usage Recommendations
  Optimal conditions to be determined by end user
• Assay Information
  This Parvovirus B19 IgM ELISA Kit is a solid phase enzymelinked immunosorbent assay (ELISA). Patient serum samples are diluted and simultaneously absorbed with Sample Diluent containing hyperimmune antihuman IgGclass antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factor. Microtiter wells as a solid phase are coated with Parvovirus B19 antigen. Diluted patient specimens and readyforuse controls are pipetted into these wells. During incubation Parvovirus B19specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated antihuman IgM antibodies are dispensed into the wells. During a second incubation this antiIgM conjugate  binds specificly to IgM antibodies resulting in the formation of enzymelinked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Parvovirus B19specific IgM antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
• URL
• More info
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

• Gene target
  Parvovirus  
• Short name
  Parvovirus IgM ELISA kit
• Technique
  ELISA kit, ELISA, IgM, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. IgMs
• Host
  antigen assay ELISA kit 1 Kit (96 Wells)
• Isotype
  IgM, IgM
• Alternative name
  ELISA kit for the detection of Parvovirus IgM in the research laboratory
• Alternative technique
  elisas, kits, immunoglobulin-m
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Virus
  parvovirus

MeSH Data

• Name
  Enzyme-Linked Immunosorbent Assay
• Concept
  Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
• Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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