Goat anti Mouse IgG1 (FITC)

• Catalog number
  43R-1648
• Price
  Please ask
• Size
  1 mg

• Applications
  ELISA, FC, IF, WB
• Concentration
  1 mg/ml
• Product Subtype
  Conjugated Secondary Antibodies
• Research Area
  Immunology
• Product Type
  Secondary Antibodies
• Immunogen
  Goat anti Mouse IgG1 was raised in Goat using pooled antisera from goats hyperimmunized with mouse IgG1 paraproteins as the immunogen.
• Specificity
  Heavy chain of mouse IgG1
• Form Buffer
  liquid form in PBS/NaN3 buffer
• Storage
  Store at 2-8 deg C
• Shipping Info
  Blue Ice
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
• Properties
  This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Latin name
  Capra aegagrus hircus, Mus musculus
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

• Gene target
  IgG1  
• Short name
  Goat anti Mouse IgG1 (FITC)
• Technique
  anti mouse, Mouse, anti, FITC, Goat, antibody to, anti mouse antibodies, Fluorescein, goats, mouses
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  IgG1, IgG1
• Label
  FITC
• Species
  Mouse, Goats, Mouses
• Alternative name
  Goat anti Mouse IgG1 secondary antibody (FITC)
• Alternative technique
  murine, antibodies, caprine, fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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