Goat anti Human IgM mu chain biotin
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Catalog number
43R-1240
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Price
Please ask
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Size
500 µg
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Purified Human IgM
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Raised in
Goat
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Specificity
This antibody reacts with heavy chains on human IgM (mu chain).
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Cross Reactivity
No reactivity is observed to non-immunoglobulin human serum proteins light chains on all human immunoglobulins human IgA + IgG.
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Conjugate Tag
Biotin
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Grade Purity
Grater than 90% pure
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Method of Purification
Affinity purified using solid phase Human IgM
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Source
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
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Concentration
1.0 mg/ml (lot specific)
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Form Buffer
Clear, colorless liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
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Storage
Store at 2-8 deg C.
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Shipping conditions
Blue Ice
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Tested for
Please refer to the datasheet
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Usage Recommendations
1:2000-1:20,000 ELISA/WB, 1:500-1:5000 IHC
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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Conjugation
Biotinylated
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Latin name
Capra aegagrus hircus
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Gene target
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Gene symbol
JCHAIN, AP2M1
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Short name
Goat anti IgM mu chain
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Technique
anti human, anti, Goat, IgM, antibody to, anti human antibodies, goats, IgMs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgM, IgM
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Label
Biotin
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Species
Human, Goats, Humans
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Alternative name
Goat anti Human IgM (mu chain) secondary antibody (biotin)
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Alternative technique
antibodies, caprine, immunoglobulin-m
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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