CaspSCREEN Flow Cytometric Apoptosis Detection Kit

Category

Research kit
Area of research

Cell Cycle & Cell Death
Form Buffer

Assay Kit containing the folllowing: D₂R Reagent, DTT (1 M), D₂R Incubation Buffer
Storage

Store at -20 deg C
Shipping conditions

Blue Ice
Tested for

FC; Functional Assay
Additional Information

CaspSCREEN is a registered trademark
URL
More info
Description

This 1 is suited for programmed cell-death studies. The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
Test

Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.

Gene target

CaspSCREEN Cytometric Kit
Short name

CaspSCREEN Cytometric Apoptosis Kit
Technique

apoptosis, detection, Flow, apoptosis pathway
Alternative name

Apoptosis Kit for use in the research laboratory
Alternative technique

celldeath, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Name

Models, Chemical
Concept

Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
Tree numbers
- E05.599.495