Biotin antibody Alk Phos
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Catalog number60R-1001
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PricePlease ask
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Size1 mg
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Polyclonal Antibodies
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Area of researchMiscellaneous
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Type of ImmunogenBiotin
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Raised inGoat
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Conjugate TagAlkaline Phosphatase
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Grade PurityGrater than 95% pure
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Method of PurificationAffinity purified using solid phase biotin
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SourceGoat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
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Concentration1.50 mg/ml (lot specific)
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Form BufferClear, colorless liquid, containing 30 mM Triethanolamine, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
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StorageStore undiluted liquid at 2-8 deg C. For storage at -20 deg C, aliquot and dilute with an equal volume of glycerol to prevent loss of enzymatic activity.
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Shipping conditionsBlue Ice
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Tested forPlease refer to the datasheet
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Usage Recommendations1:5000-1:50,000 ELISA/WB, 1:500-1:2000 IHC
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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French translationanticorps
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Gene target
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Short nameantibody Alk Phos
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TechniqueAntibody, antibodies against human proteins, antibodies for
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LabelBiotin
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Alternative nameBiotin antibody (Alk Phos)
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Alternative techniqueantibodies
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Alternative to gene targetanaplastic lymphoma receptor tyrosine kinase, CD246 and NBLST3, ALK and IDBG-42084 and ENSG00000171094 and 238, transferase activity, Plasma membranes, Alk and IDBG-197259 and ENSMUSG00000055471 and 11682, ALK and IDBG-636042 and ENSBTAG00000007379 and 536642
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data