AKR1C3 protein His tag

  • Catalog number
    80R-1116
  • Price
    Please ask
  • Size
    100 µg
  • Category
    Proteins
  • Antibody Subtype
    Conjugated Proteins
  • Area of research
    Signal Transduction
  • Reactivity
    Human
  • Residues
    1-323 amino acids: MGSSHHHHHH SSGLVPRGSH MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD RNLHYFNSDS FASHPNYPYS DEY
  • Conjugate Tag
    His tag
  • Type of protein
    Recombinant
  • Expression System
    Escherichia coli
  • Grade Purity
    Grater than 95% pure
  • Concentration
    1 mg/ml (lot specific)
  • Form Buffer
    Provided as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.
  • Storage
    Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
  • Shipping conditions
    Blue Ice
  • Tested for
    SDS-PAGE
  • Bioactivity
    Specific activity: approximately < 0.1 units/mg. Ezymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH per minute at 25 Deg C.
  • Assay Information
    1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 0.1M sodium phosphate (pH 7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50ul of recombinant AKR1C3 solution with various concentrations (2.5ug, 5ug, 10ug) in 750ul reaction buffer. 3. Mix by inversion and Incubate at 25C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase at A340nm for 3 minutes.
  • Additional Information
    AKR1C3 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
  • URL
  • Conjugation
    histidine
  • Gene target
    AKR1C3   protein   His   tag  
  • Gene symbol
    AKR1C3
  • Short name
    AKR1C3 protein His tag
  • Technique
    Tags
  • Label
    His
  • Alternative name
    Purified recombinant Human AKR1C3 protein
  • Alternative to gene target
    aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II), DD3 and DDX and HA1753 and HAKRB and HAKRe and hluPGFS and HSD17B5 and PGFS, AKR1C3 and IDBG-47964 and ENSG00000196139 and 8644, oxidoreductase activity, nuclei
Gene info
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