AKR1C3 protein His tag
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Catalog number80R-1116
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PricePlease ask
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Size100 µg
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CategoryProteins
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Antibody SubtypeConjugated Proteins
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Area of researchSignal Transduction
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ReactivityHuman
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Residues1-323 amino acids: MGSSHHHHHH SSGLVPRGSH MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD RNLHYFNSDS FASHPNYPYS DEY
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Conjugate TagHis tag
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Type of proteinRecombinant
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Expression SystemEscherichia coli
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Grade PurityGrater than 95% pure
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Concentration1 mg/ml (lot specific)
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Form BufferProvided as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.
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StorageStore at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forSDS-PAGE
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BioactivitySpecific activity: approximately < 0.1 units/mg. Ezymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH per minute at 25 Deg C.
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Assay Information1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 0.1M sodium phosphate (pH 7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50ul of recombinant AKR1C3 solution with various concentrations (2.5ug, 5ug, 10ug) in 750ul reaction buffer. 3. Mix by inversion and Incubate at 25C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase at A340nm for 3 minutes.
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Additional InformationAKR1C3 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Conjugationhistidine
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Gene target
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Gene symbolAKR1C3
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Short nameAKR1C3 protein His tag
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TechniqueTags
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LabelHis
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Alternative namePurified recombinant Human AKR1C3 protein
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Alternative to gene targetaldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II), DD3 and DDX and HA1753 and HAKRB and HAKRe and hluPGFS and HSD17B5 and PGFS, AKR1C3 and IDBG-47964 and ENSG00000196139 and 8644, oxidoreductase activity, nuclei
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Gene info
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Identity
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Gene
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Long gene namealdo-keto reductase family 1 member C3
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Synonyms gene
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Synonyms gene name
- hydroxysteroid (17-beta) dehydrogenase 5
- aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-09-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Aldo-keto reductases
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VEGA ID