anti- AFF1-Specific antibody

• Catalog number
  FNab00195
• Price
  Please ask
• Size
  2X100μg

• Form
  liquid
• Purification
  Immunogen affinity purified
• Purity
  ≥95% as determined by SDS-PAGE
• Storage
  PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
• Background
  This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants.
• Immunogen
  AF4/FMR2 family, member 1
• Synonyms
  AFF1, MLLT2, AF4 MLL, PBM1, AF 4
• Calculated MW
  Refer to figures
• Uniprot ID
  P51825
• Specificity
  Human
• Tested Application
  ELISA, WB, IHC
• Recommended dilution
  WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:100
• Image4
  K-562 cells were subjected to SDS PAGE followed by western blot with FNab00195(AFF1-Specific antibody) at dilution of 1:1000
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  AFF1-Specific  
• Gene symbol
  AFF1-AS1, AFF1
• Short name
  anti- AFF1-Specific antibody
• Technique
  Antibody, anti, antibody to, antibodies against human proteins, antibodies for
• Host
  Rabbit
• Isotype
  IgG
• Alternative name
  antibody to- AFF1-Specific (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:53447
• Gene
  AFF1-AS1
• Long gene name
  AFF1 antisense RNA 1
• Locus
  4q21.3
• Discovery year
  2017-04-19
• Entrez gene record
  100506746
• RefSeq identity
  • NR_038841
• Classification
  • Antisense RNAs

Gene info

• Identity
  HGNC:7135
• Gene
  AFF1
• Long gene name
  AF4/FMR2 family member 1
• Synonyms gene
  • PBM1
  • MLLT2
• Synonyms gene name
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog); translocated to, 2
  • pre-B-cell monocytic leukemia partner 1
  • AF4/FMR2 family, member 1
• Synonyms
  • AF-4
  • AF4
• GenBank acession
  • L22179
• Locus
  4q21.3-q22.1
• Discovery year
  2005-06-27
• Entrez gene record
  4299
• Pubmed identfication
  • 7689231
  • 1423625
  • 8353274
• RefSeq identity
  • NM_005935
• Classification
  • Super elongation complex
  • AF4/FMR2 family
• VEGA ID
  OTTHUMG00000130603

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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