gentaur logogentaur logo
x

Mouse IgG2a Isotype Control

  • Catalog number
    A4-458-C100
  • Product name
    Mouse IgG2a Isotype Control
  • Size
    100ug
  • Short name
    IgG2a Isotype Control
  • Long name
    Mouse Monoclonal IgG2a Isotype Control
  • Antigen
    Mouse IgG2a Isotype Control
  • Host
    Mouse (Mus musculus)
  • Species reactivity
    Immunoglobulin Isotype Control
  • Negative species reactivity
    Human, Other species Not tested (expected negative), Porcine, Mouse, Rat
  • Clonality
    Monoclonal Antibody (Mab)
  • Clone
    PPV-04
  • Isotype
    IgG2a, IgG2a
  • Applications
    FC, negative control
  • Immunogen
    Ig Control
  • Format conjugation
    Alexa Fluor 488
  • Specificity
    This mouse IgG2a monoclonal antibody (clone PPV-04) reacts with undefined epitope on a plant pathogen.
  • Research area
    Immunoglobulin Isotype Controls
  • Concentration
    1 mg/ml
  • Storage buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  • Shipping and storage
    Mouse Monoclonal IgG2a Isotype Control is shipped on blue ice or ice packs. Upon receipt store at 4 degrees Celcius. Do not freeze!
  • Background
    The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-04, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
  • Purity
    > 95% (by SDS-PAGE)
  • Purification method
    Purified by affinity chromatography
  • Datasheet
    Datasheet
  • References
    Contact us for the most recent list of references
  • Gene target
    IgG2a
  • isotype filter
    • IgG2a
  • technique filter
    • Control
    • Isotype
    • Mouse
  • Technique
    Control, Isotype, Mouse, controls, Isotypes and controls, mouses
  • Alternative technique
    control, controls, murine
  • Tissue
    control
  • tissue filter
    • control