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Long name
Hamster Monoclonal Antibody against Mouse Bim, Clone: Ham151-149
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Antigen
Bim (mouse)
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Species reactivity
Mouse (Mus musculus)
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
Ham151-149
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Applications
FC, IP, WB, ICC
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Immunogen
Mouse BimL (w/o transmembrane region) + Bcl-xL (w/o transmembrane region)-6-his tag, purified from Hi5 cells_x000D_
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Format conjugation
purified; unconjugated
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Specificity
The Armenian hamster antibody Ham151-149 reacts with mouse Bim, a 19-24 kDa pro-apoptotic protein (Bcl-2 family) which regulates immunological responses._x000D_
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Research area
Apoptosis (Rodent)
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Hamster Monoclonal Antibody against Mouse Bim, Clone: Ham151-149 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
Bim (Bcl2-interacting mediator) is a pro-apoptotic protein of BH3 domain-only subgroup of the Bcl2 family. It has important roles in initiation of apoptosis in response to many death stimuli. Bim is an important regulator of B and T cell negative selection and is also an essential regulator of T cell apoptosis during termination of an immune response. Bim is constitutively expressed in many cell types but it is maintained in an inactive form through binding to the microtubule-associated dynein motor complex._x000D_
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Jorgensen TN, McKee A, Wang M, Kushnir E, White J, Refaeli Y, Kappler JW, Marrack P: Bim and Bcl-2 mutually affect the expression of the other in T cells. J Immunol. 2007 Sep 15;179(6):3417-24., *Oliver PM, Wang M, Zhu Y, White J, Kappler J, Marrack P: Loss of Bim allows precursor B cell survival but not precursor B cell differentiation in the absence of interleukin 7. J Exp Med. 2004 Nov 1;200(9):1179-87._x000D_
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps