5-bromodeoxyuridine monoclonal antibody
#
-
Catalog number1P-682-T100
-
Price:
-
Size100 assays
-
-
Long nameMouse Monoclonal Antibody against All species 5-bromodeoxyuridine, Clone: Bu20a
-
Antigen5-bromodeoxyuridine (BrdU)
-
Negative species reactivityat the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
-
ClonalityMonoclonal Antibody (Mab)
-
CloneBu20a
-
ApplicationsFC, IHC(P), IHC(F)
-
ImmunogenBromodeoxyuridine conjugated to BSA
-
Format conjugationPE
-
SpecificityThe antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining.
-
Research areaSmall Organic Molecules
-
Storage bufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
-
Shipping and storageMouse Monoclonal Antibody against All species 5-bromodeoxyuridine, Clone: Bu20a is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
-
BackgroundBromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.
-
Purity> 95% (by SDS-PAGE)
-
Purification methodPurified by affinity chromatography
-
References, *Magaud JP, Sargent I, Clarke PJ, Ffrench M, Rimokh R, Mason DY: Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem. 1989 Oct;37(10):1517-27., *Yatera K, Hsieh J, Hogg JC, Tranfield E, Suzuki H, Shih CH, Behzad AR, Vincent R, van Eeden SF: Particulate matter air pollution exposure promotes recruitment of monocytes into _x000D_ atherosclerotic plaques. Am J Physiol Heart Circ Physiol. 2008 Feb;294(2):H944-53., *Martín CA, Surur JM, García MN, Corrons F, Badrán AF: Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver. Biocell. 2005 Apr;29(1):33-8., *Shih CH, Whalen BA, Goto Y, Hogg JC, van Eeden SF: Flow cytometric method for enumeration and characterization of newly released polymorphonuclear leukocytes from the bone marrow using 5'-bromo-2'-deoxyuridine. Am J Physiol Cell Physiol. 2005 Sep;289(3):C757-65., *Lagares A, Li HY, Zhou XF, Avendaño C: Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence_x000D_ for neural crest glio-neuronal precursor maturation. J Neurosci. 2007 Jul 25;27(30):7939-53., *Muskhelishvili L, Latendresse JR, Kodell RL, Henderson EB: Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67(MIB-5) immunohistochemistry and in situ hybridization for histone mRNA. J Histochem Cytochem. 2003 Dec;51(12):1681-8., *Pajalunga D, Mazzola A, Salzano AM, Biferi MG, De Luca G, Crescenzi M: Critical requirement for cell cycle inhibitors in sustaining nonproliferative states. J Cell Biol. 2007 Mar 12;176(6):807-18., O'Driscoll M, Jeggo PA: CsA can induce DNA double-strand breaks: implications for BMT regimens_x000D_ particularly for individuals with defective DNA repair. Bone Marrow Transplant. 2008 Jun;41(11):983-9.
-
PropertiesIf you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
French translationanticorps
-
Gene target
-
Short nameAnti-5-bromodeoxyuridine Mab
-
TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
-
HostMouse (Mus musculus)
-
IsotypeIgG1
-
Alternative nameMouse Monoclonal to 5-bromodeoxyuridine (BrdU)
-
Alternative techniqueantibodies
-
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data