-
Host Source
Rabbit
-
Clone Name
Polyclonal
-
Product Category
Veterinary
-
Species Reactivity
Mouse
-
Application
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
-
Immunogen
Purified homogenous IgM isolated from mouse serum. Immunization with intact (19S) and split IgM (7S). FreundÍs complete adjuvant is used in the first step of the immunization procedure.
-
Purification Method
NA
-
Shipping Conditions
NA
-
Storage conditions
Rabbit anti Mouse IgM (Fc specific), conjugated with Biotin, Secondary Antibodies philized conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4ÁC--at least 10 years. Reconstituted at or below -20ÁC--3-5 years. Reconstituted at +4ÁC--7 days
-
Datasheet link
NA
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
-
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
-
Conjugation
Biotinylated
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus, Oryctolagus cuniculus
-
About
Rabbits are used for polyclonal antibody production by Exalpha. Rabbit antibodies are very stable and can be stored for several days at room temperature. Exalpha adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Gene target
-
Short name
Rabbit anti Mouse IgM (Fc specific), conjugated with , Secondary Antibodies
-
Technique
anti mouse, Rabbit, Mouse, anti, IgM, FC, antibody to, anti mouse antibodies, antibody Conjugates, IgMs, mouses
-
Host
Rabbit, Rabbits
-
Isotype
NA, IgM
-
Label
Biotin
-
Species
Mouse, Mouses
-
Alternative name
production species: rabbit antibody to Mouse Immunoglobulin M (fragment c specific), coupled including biotinilated, secondary detector Antibody Antibodies
-
Alternative technique
rabbit-anti, murine, antibodies, immunoglobulin-m
-