ClonoPath(tm) - CD38/CD56/CD19, Flow Cytometry Kits

Host Source

Mouse
Clone Name

NA
Product Category

Flow Cytometry
Species Reactivity

NA
Application

Flow Cytometry
Immunogen

NA
Purification Method

NA
Shipping Conditions

Room Temperature
Storage conditions

ClonoPath(tm) - CD38/CD56/CD19, Flow Cytometry Kits should be stored at 4-8ºC. DO NOT FREEZE
Datasheet link
Description

FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors.
Test

Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.

Gene target

ClonoPath CD38/CD56/CD19
Gene symbol

CD19, CD38, NCAM1
Short name

ClonoPath(tm) - CD38/CD56/CD19,
Technique

Flow Cytometry, Flow
Isotype

NA
Alternative name

ClonoPath(tm) - CD38 molecule/CD56/CD19 molecule, Flow Cytometry reagens
Alternative to gene target

CD38 molecule, ADPRC 1 and T10, CD38 and IDBG-10668 and ENSG00000004468 and 952, hydrolase activity, nuclei, Cd38 and IDBG-161475 and ENSMUSG00000029084 and 12494, CD38 and IDBG-643982 and ENSBTAG00000013569 and 327677

Identity

HGNC:1633
Gene

CD19
Long gene name

CD19 molecule
Synonyms gene name
- CD19 antigen
Locus

16p11.2
Discovery year

1991-06-04
Entrez gene record

930
Classification
- CD molecules
- Immunoglobulin like domain containing
- Minor histocompatibility antigens
VEGA ID

OTTHUMG00000097049
Locus Specific Databases
- CD19base: Mutation registry for CD19 deficiency
- LRG_35

Identity

HGNC:7656
Gene

NCAM1
Long gene name

neural cell adhesion molecule 1
Synonyms
- NCAM
- CD56
Locus

11q23.2
Discovery year

2001-06-22
Entrez gene record

4684
RefSeq identity
- NM_000615
Classification
- Fibronectin type III domain containing
- CD molecules
- Ig-like cell adhesion molecule family
- I-set domain containing
VEGA ID

OTTHUMG00000167196

Name

Flow Cytometry
Concept

Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data