CD8 Biotin, Single-Color Reagent

Host Source

Mouse
Clone Name

17D8
Product Category

Flow Cytometry
Species Reactivity

Human
Application

Flow Cytometry
Immunogen

CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
Purification Method

Protein A/G Chromatography
Shipping Conditions

Room Temperature
Storage conditions

CD8 Biotin, Single-Color Reagent should be stored at 4-8ºC. DO NOT FREEZE
Datasheet link
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
Description

Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.

Identity

HGNC:1706
Gene

CD8A
Long gene name

CD8a molecule
Synonyms gene
- CD8
Synonyms gene name
- CD8 antigen, alpha polypeptide (p32)
- T-cell surface glycoprotein CD8 alpha chain
Locus

2p11.2
Discovery year

1986-01-01
Entrez gene record

925
Pubmed identfication
- 1541829
RefSeq identity
- NM_001768
Classification
- V-set domain containing
- CD molecules
VEGA ID

OTTHUMG00000130265
Locus Specific Databases
- CD8Abase: Mutation registry for CD8 deficiency
- LRG_44