WI-38 Cell Line
-
Catalog numberEP-CL-0243
-
PricePlease ask
-
Size1×10⁶ cells/vial
-
-
AbbreviationWI-38
-
Leadtime3-5 days
-
Shipping conditionDry ice
-
StorageLiquid nitrogen -196°C
-
AgeEmbryo
-
MorphologyFibroblast
-
Growth_propertiesAdherent
-
Doubling_timeNA
-
BackgroundNA
-
Biosafety1
-
MediumMEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
-
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
-
Ratio1:2-1:4
-
RenewalEvery 2 to 3 days
-
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
-
Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
-
TumorigenicNA
-
EffectsNA
-
Receptor_expressionNA
-
Antigen_expressionNA
-
Gene_expressionNA
-
ApplicationsNA
-
DurationNA
-
DescriptionFor cells, cell lines and tissues in culture till half confluency.
-
Gene target
-
Gene symbolIGLVI-38, SNORD115-38, IGHV3-38, IGKV2D-38, GPR75, IGKV2-38, IGHVIII-38-1, RNU6-38P, TPPP3, IGHV4-38-2
-
Short nameWI-38 Line
-
SpeciesHomo sapiens, Human
-
Alternative nameWI-38 cellular Line
-
TissueEmbryo
-
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin lambda variable (I)-38 (pseudogene)
-
Synonyms gene name
- immunoglobulin lambda variable (I)-38
- immunoglobulin lambda variable (I)-38 pseudogene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-05-08
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin lambda locus at 22q11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 115-38
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2007-02-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable 3-38 (non-functional)
-
Synonyms gene name
- immunoglobulin heavy variable 3-38
-
GenBank acession
-
Locus
-
Discovery year2000-04-04
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 2D-38 (pseudogene)
-
Synonyms gene name
- immunoglobulin kappa variable 2D-38
- immunoglobulin kappa variable 2D-38 pseudogene
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin kappa locus at 2p11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameG protein-coupled receptor 75
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1999-10-22
-
Entrez gene record
-
Pubmed identfication
-
Classification
- G protein-coupled receptors, Class A orphans
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 2-38 (pseudogene)
-
Synonyms gene name
- immunoglobulin kappa variable 2-38
- immunoglobulin kappa variable 2-38 pseudogene
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin kappa locus at 2p11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable (III)-38-1 (pseudogene)
-
Synonyms gene name
- immunoglobulin heavy variable (III)-38-1
- immunoglobulin heavy variable (III)-38-1 pseudogene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-17
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameRNA, U6 small nuclear 38, pseudogene
-
Synonyms gene
-
Synonyms gene name
- RNA, U6 small nuclear 38
-
Locus
-
Discovery year2008-06-10
-
Entrez gene record
Gene info
-
Identity
-
Gene
-
Long gene nametubulin polymerization promoting protein family member 3
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2007-05-02
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Tubulin polymerization promoting proteins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable 4-38-2
-
Synonyms gene
-
Synonyms gene name
- immunoglobulin heavy variable 4-B (provisional)
-
GenBank acession
-
Locus
-
Discovery year2000-04-04
-
Entrez gene record
-
Classification
- Immunoglobulin heavy locus at 14q32.33