UMNSAH/DF-1 Cell Line

  • Catalog number
    EP-CL-0279
  • Price
    Please ask
  • Size
    1×10⁶ cells/vial
  • Abbreviation
    UMNSAH/DF-1
  • Leadtime
    3-5 days
  • Shipping condition
    Dry ice
  • Storage
    Liquid nitrogen -196°C
  • Age
    10 days gestation
  • Morphology
    Fibroblast
  • Growth_properties
    Adherent
  • Doubling_time
    NA
  • Background
    Foci of non-senescent cells were identified and grown for greater than 30 passages.No clonal proliferation was observed in soft agar cultures, indicating that these cells were immortalized but not transformed.
  • Biosafety
    1
  • Medium
    DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
  • Subculturing
    Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
  • Ratio
    1:2-1:4
  • Renewal
    Every 2 to 3 days
  • Cryopreservation
    Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
  • Culture_conditions
    Atmosphere: Air, 95%; CO2, 5% Temperature: 39℃(Max.40℃, Min.38℃)
  • Tumorigenic
    No
  • Effects
    No, in immunosuppressed mice; No, in semisolid medium.
  • Receptor_expression
    NA
  • Antigen_expression
    NA
  • Gene_expression
    NA
  • Applications
    The cells are useful as substrates for virus propagation, recombinant protein expression and recombinant virus production.
  • Duration
    NA
  • Description
    For cells, cell lines and tissues in culture till half confluency.
  • Gene target
  • Gene symbol
    CFD, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1, MIR1289-1, MIR16-1
  • Short name
    UMNSAH/DF-1 Line
  • Species
    Gallus gallus, Chicken
  • Alternative name
    UMNSAH/DF-1 cellular Line
  • Tissue
    Embryo
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (II)-1-1 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (II)-1-1
    • immunoglobulin heavy variable (II)-1-1 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-undetermined (NNN) 8-1
  • Synonyms gene name
    • transfer RNA-undetermined (NNN) 8-1
  • GenBank acession
  • Locus
    1
  • Discovery year
    2014-06-20
  • Entrez gene record
  • Pubmed identfication
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-undetermined (NNN) 7-1
  • Synonyms gene name
    • transfer RNA-undetermined (NNN) 7-1
  • GenBank acession
  • Locus
    1
  • Discovery year
    2014-06-20
  • Entrez gene record
  • Pubmed identfication
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin kappa variable 1/OR2-1 (pseudogene)
  • Synonyms gene
  • Synonyms gene name
    • immunoglobulin kappa variable 1/OR-1
    • immunoglobulin kappa variable 1/OR-1 pseudogene
    • immunoglobulin kappa variable 1/OR-1 (pseudogene)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-18
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Immunoglobulin kappa (IGK) orphons
  • VEGA ID
Gene info
Gene info
Gene info
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