UM-UC-3 Cell Line
-
Catalog numberEP-CL-0463
-
PricePlease ask
-
Size1×10⁶ cells/vial
-
-
AbbreviationUM-UC-3
-
Leadtime3-5 days
-
Shipping conditionDry ice
-
StorageLiquid nitrogen -196°C
-
AgeNA
-
MorphologyEpithelial
-
Growth_propertiesAdherent
-
Doubling_timeNA
-
BackgroundNA
-
Biosafety1
-
MediumMEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
-
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
-
Ratio1:2-1:4
-
RenewalEvery 2 to 3 days
-
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
-
Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
-
TumorigenicYes
-
EffectsYes, Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
-
Receptor_expressionNA
-
Antigen_expressionNA
-
Gene_expressionNA
-
ApplicationsNA
-
DurationNA
-
DescriptionFor cells, cell lines and tissues in culture till half confluency.
-
Gene target
-
Gene symbolPCBP2-OT1, MIR1302-3, MIR509-3, MIR7-3, PIRC14, PIRC12, MIR9-3, MIR941-3
-
Short nameUM-UC-3 Line
-
SpeciesHomo sapiens, Human
-
Alternative nameUM-UC-3 cellular Line
-
TissueUrinary bladder
-
Gene info
-
Identity
-
Gene
-
Long gene namePCBP2 overlapping transcript 1
-
Synonyms gene name
- PCBP2 overlapping transcript 1 (non-protein coding)
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2013-09-16
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Overlapping transcripts
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 1302-3
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2008-11-03
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR1302 family
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 509-3
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2007-10-24
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR506 family
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 7-3
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR7 family
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 14
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 12
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 9-3
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR9 family
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 941-3
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2007-10-24
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNAs